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Journal of Clinical Microbiology, July 2008, p. 2399-2401, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00024-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Quadruplex PCR for Simultaneous Detection of Serotype, Biotype, Toxigenic Potential, and Central Regulating Factor of Vibrio cholerae

Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar, Pin-751023, Orissa, India

Received 7 January 2008/ Returned for modification 11 February 2008/ Accepted 30 April 2008

A quadruplex PCR was developed for the simultaneous detection of genes specific for Vibrio cholerae O1 and/or O139 serogroup (wbe and/or wbf), cholera toxin A subunit (ctxA), toxin-coregulated pilus (tcpA), and central regulating protein ToxR (toxR) in a single tube reaction. This is a simple, rapid, and accurate approach for the detection of toxigenic V. cholerae O1 and/or O139 and can prevent the rapid spread of the disease by early detection.

Published ahead of print on 7 May 2008.