Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR [published erratum appears in J Clin Microbiol 1995 May;33(5):1434]

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Dutka-Malen, S.
	Articles by Courvalin, P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Dutka-Malen, S.
	Articles by Courvalin, P.

Previous Article | Next Article

Journal of Clinical Microbiology, Jan 1995, 24-27, Vol 33, No. 1
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR [published erratum appears in J Clin Microbiol 1995 May;33(5):1434]

S Dutka-Malen, S Evers and P Courvalin
Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.

A PCR assay that allows simultaneous detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci (Enterococcus faecium, E. faecalis, E. gallinarum, and E. casseliflavus) was developed. This assay was based on specific amplification of internal fragments of genes encoding D- alanine:D-alanine ligases and related glycopeptide resistance proteins. The specificity of the assay was tested on 5 well-characterized glycopeptide-resistant strains and on 15 susceptible enterococcal type strains. Clinical isolates of enterococci that could not be identified to the species level by conventional methods were identified by the PCR test. This assay offers a specific and rapid alternative to antibiotic susceptibility tests, in particular for detection of low-level vancomycin resistance.

This article has been cited by other articles:

Boyd, D. A., Willey, B. M., Fawcett, D., Gillani, N., Mulvey, M. R. (2008). Molecular Characterization of Enterococcus faecalis N06-0364 with Low-Level Vancomycin Resistance Harboring a Novel D-Ala-D-Ser Gene Cluster, vanL. Antimicrob. Agents Chemother. 52: 2667-2672 [Abstract] [Full Text]
Grabsch, E. A., Chua, K., Xie, S., Byrne, J., Ballard, S. A., Ward, P. B., Grayson, M. L. (2008). Improved Detection of vanB2-Containing Enterococcus faecium with Vancomycin Susceptibility by Etest Using Oxgall Supplementation. J. Clin. Microbiol. 46: 1961-1964 [Abstract] [Full Text]
Ergani-Ozcan, A., Naas, T., Baysan, B. O., Ogunc, D., Inan, D., Colak, D., Nordmann, P. (2008). Nosocomial outbreak of vancomycin-resistant Enterococcus faecium in a paediatric unit at a Turkish university hospital. J Antimicrob Chemother 61: 1033-1039 [Abstract] [Full Text]
Graham, M., Ballard, S. A., Grabsch, E. A., Johnson, P. D. R., Grayson, M. L. (2008). High Rates of Fecal Carriage of Nonenterococcal vanB in both Children and Adults. Antimicrob. Agents Chemother. 52: 1195-1197 [Abstract] [Full Text]
Top, J., Willems, R., van der Velden, S., Asbroek, M., Bonten, M. (2008). Emergence of Clonal Complex 17 Enterococcus faecium in The Netherlands. J. Clin. Microbiol. 46: 214-219 [Abstract] [Full Text]
Deplano, A., Denis, O., Nonhoff, C., Rost, F., Byl, B., Jacobs, F., Vankerckhoven, V., Goossens, H., Struelens, M. J. (2007). Outbreak of hospital-adapted clonal complex-17 vancomycin-resistant Enterococcus faecium strain in a haematology unit: role of rapid typing for early control. J Antimicrob Chemother 60: 849-854 [Abstract] [Full Text]
Biavasco, F., Foglia, G., Paoletti, C., Zandri, G., Magi, G., Guaglianone, E., Sundsfjord, A., Pruzzo, C., Donelli, G., Facinelli, B. (2007). VanA-Type Enterococci from Humans, Animals, and Food: Species Distribution, Population Structure, Tn1546 Typing and Location, and Virulence Determinants. Appl. Environ. Microbiol. 73: 3307-3319 [Abstract] [Full Text]
Moritz, E. M., Hergenrother, P. J. (2007). Toxin-antitoxin systems are ubiquitous and plasmid-encoded in vancomycin-resistant enterococci. Proc. Natl. Acad. Sci. USA 104: 311-316 [Abstract] [Full Text]
Weigel, L. M., Donlan, R. M., Shin, D. H., Jensen, B., Clark, N. C., McDougal, L. K., Zhu, W., Musser, K. A., Thompson, J., Kohlerschmidt, D., Dumas, N., Limberger, R. J., Patel, J. B. (2007). High-Level Vancomycin-Resistant Staphylococcus aureus Isolates Associated with a Polymicrobial Biofilm. Antimicrob. Agents Chemother. 51: 231-238 [Abstract] [Full Text]
Perichon, B., Courvalin, P. (2006). Synergism between {beta}-Lactams and Glycopeptides against VanA-Type Methicillin-Resistant Staphylococcus aureus and Heterologous Expression of the vanA Operon. Antimicrob. Agents Chemother. 50: 3622-3630 [Abstract] [Full Text]
Abele-Horn, M., Vogel, U., Klare, I., Konstabel, C., Trabold, R., Kurihara, R., Witte, W., Kreth, W., Schlegel, P.-G., Claus, H. (2006). Molecular Epidemiology of Hospital-Acquired Vancomycin-Resistant Enterococci. J. Clin. Microbiol. 44: 4009-4013 [Abstract] [Full Text]
Hasman, H., Kempf, I., Chidaine, B., Cariolet, R., Ersboll, A. K., Houe, H., Bruun Hansen, H. C., Aarestrup, F. M. (2006). Copper Resistance in Enterococcus faecium, Mediated by the tcrB Gene, Is Selected by Supplementation of Pig Feed with Copper Sulfate. Appl. Environ. Microbiol. 72: 5784-5789 [Abstract] [Full Text]
Brigante, G., Luzzaro, F., Bettaccini, A., Lombardi, G., Meacci, F., Pini, B., Stefani, S., Toniolo, A. (2006). Use of the Phoenix Automated System for Identification of Streptococcus and Enterococcus spp.. J. Clin. Microbiol. 44: 3263-3267 [Abstract] [Full Text]
Torres, C., Escobar, S., Portillo, A., Torres, L., Rezusta, A., Ruiz-Larrea, F., Revillo, M. J., Aspiroz, C., Zarazaga, M. (2006). Detection of clonally related vanB2-containing Enterococcus faecium strains in two Spanish hospitals.. J Med Microbiol 55: 1237-1243 [Abstract] [Full Text]
Macovei, L., Zurek, L. (2006). Ecology of antibiotic resistance genes: characterization of enterococci from houseflies collected in food settings.. Appl. Environ. Microbiol. 72: 4028-4035 [Abstract] [Full Text]
Batista Xavier, D., Moreno Bernal, F. E., Titze-de-Almeida, R. (2006). Absence of VanA- and VanB-Containing Enterococci in Poultry Raised on Nonintensive Production Farms in Brazil. Appl. Environ. Microbiol. 72: 3072-3073 [Abstract] [Full Text]
Arias, C. A., Robredo, B., Singh, K. V., Torres, C., Panesso, D., Murray, B. E. (2006). Rapid Identification of Enterococcus hirae and Enterococcus durans by PCR and Detection of a Homologue of the E. hirae mur-2 Gene in E. durans. J. Clin. Microbiol. 44: 1567-1570 [Abstract] [Full Text]
Leavis, H. L., Willems, R. J. L., Top, J., Bonten, M. J. M. (2006). High-Level Ciprofloxacin Resistance from Point Mutations in gyrA and parC Confined to Global Hospital-Adapted Clonal Lineage CC17 of Enterococcus faecium.. J. Clin. Microbiol. 44: 1059-1064 [Abstract] [Full Text]
Naser, S. M., Vancanneyt, M., Hoste, B., Snauwaert, C., Vandemeulebroecke, K., Swings, J. (2006). Reclassification of Enterococcus flavescens Pompei et al. 1992 as a later synonym of Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984 and Enterococcus saccharominimus Vancanneyt et al. 2004 as a later synonym of Enterococcus italicus Fortina et al. 2004. Int. J. Syst. Evol. Microbiol. 56: 413-416 [Abstract] [Full Text]
Abele-Horn, M., Hommers, L., Trabold, R., Frosch, M. (2006). Validation of VITEK 2 Version 4.01 Software for Detection, Identification, and Classification of Glycopeptide-Resistant Enterococci. J. Clin. Microbiol. 44: 71-76 [Abstract] [Full Text]
Kuhn, I., Iversen, A., Finn, M., Greko, C., Burman, L. G., Blanch, A. R., Vilanova, X., Manero, A., Taylor, H., Caplin, J., Dominguez, L., Herrero, I. A., Moreno, M. A., Mollby, R. (2005). Occurrence and Relatedness of Vancomycin-Resistant Enterococci in Animals, Humans, and the Environment in Different European Regions. Appl. Environ. Microbiol. 71: 5383-5390 [Abstract] [Full Text]
Bywater, R., McConville, M., Phillips, I., Shryock, T. (2005). The susceptibility to growth-promoting antibiotics of Enterococcus faecium isolates from pigs and chickens in Europe. J Antimicrob Chemother 56: 538-543 [Abstract] [Full Text]
Naas, T., Fortineau, N., Snanoudj, R., Spicq, C., Durrbach, A., Nordmann, P. (2005). First Nosocomial Outbreak of Vancomycin-Resistant Enterococcus faecium Expressing a VanD-Like Phenotype Associated with a vanA Genotype. J. Clin. Microbiol. 43: 3642-3649 [Abstract] [Full Text]
Novais, C., Sousa, J. C., Coque, T. M., Peixe, L. V., The Portuguese Resistance Study Group, (2005). Molecular Characterization of Glycopeptide-Resistant Enterococcus faecium Isolates from Portuguese Hospitals. Antimicrob. Agents Chemother. 49: 3073-3079 [Abstract] [Full Text]
Garcia-Migura, L., Pleydell, E., Barnes, S., Davies, R. H., Liebana, E. (2005). Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Broiler Poultry and Pig Farms in England and Wales. J. Clin. Microbiol. 43: 3283-3289 [Abstract] [Full Text]
Lavigne, J.-P., Marchandin, H., Bouziges, N., Sotto, A. (2005). First Infection with VanD-Type Glycopeptide-Resistant Enterococcus faecium in Europe. J. Clin. Microbiol. 43: 3512-3515 [Abstract] [Full Text]
Naser, S. M., Thompson, F. L., Hoste, B., Gevers, D., Dawyndt, P., Vancanneyt, M., Swings, J. (2005). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151: 2141-2150 [Abstract] [Full Text]
Novais, C., Coque, T. M., Ferreira, H., Sousa, J. C., Peixe, L. (2005). Environmental Contamination with Vancomycin-Resistant Enterococci from Hospital Sewage in Portugal. Appl. Environ. Microbiol. 71: 3364-3368 [Abstract] [Full Text]
Eigner, U., Fahr, A., Weizenegger, M., Witte, W. (2005). Evaluation of a New Molecular System for Simultaneous Identification of Four Enterococcus Species and Their Glycopeptide Resistance Genotypes. J. Clin. Microbiol. 43: 2920-2922 [Abstract] [Full Text]
Petsaris, O., Miszczak, F., Gicquel-Bruneau, M., Perrin-Guyomard, A., Humbert, F., Sanders, P., Leclercq, R. (2005). Combined Antimicrobial Resistance in Enterococcus faecium Isolated from Chickens. Appl. Environ. Microbiol. 71: 2796-2799 [Abstract] [Full Text]
Naser, S., Thompson, F. L., Hoste, B., Gevers, D., Vandemeulebroecke, K., Cleenwerck, I., Thompson, C. C., Vancanneyt, M., Swings, J. (2005). Phylogeny and Identification of Enterococci by atpA Gene Sequence Analysis. J. Clin. Microbiol. 43: 2224-2230 [Abstract] [Full Text]
Reviriego, C., Eaton, T., Martin, R., Jimenez, E., Fernandez, L., Gasson, M. J., Rodriguez, J. M. (2005). Screening of Virulence Determinants in Enterococcus faecium Strains Isolated From Breast Milk. J Hum Lact 21: 131-137 [Abstract]
Kilic, A., Baysallar, M., Bahar, G., Kucukkaraaslan, A., Cilli, F., Doganci, L. (2005). Evaluation of the EVIGENE VRE Detection kit for detection of vanA and vanB genes in vancomycin-resistant enterococci. J Med Microbiol 54: 347-350 [Abstract] [Full Text]
Duckro, A. N., Blom, D. W., Lyle, E. A., Weinstein, R. A., Hayden, M. K. (2005). Transfer of Vancomycin-Resistant Enterococci via Health Care Worker Hands. Arch Intern Med 165: 302-307 [Abstract] [Full Text]
Ballard, S. A., Grabsch, E. A., Johnson, P. D. R., Grayson, M. L. (2005). Comparison of Three PCR Primer Sets for Identification of vanB Gene Carriage in Feces and Correlation with Carriage of Vancomycin-Resistant Enterococci: Interference by vanB-Containing Anaerobic Bacilli. Antimicrob. Agents Chemother. 49: 77-81 [Abstract] [Full Text]
Palazzo, I. C. V., Araujo, M. L. C., Darini, A. L. C. (2005). First Report of Vancomycin-Resistant Staphylococci Isolated from Healthy Carriers in Brazil. J. Clin. Microbiol. 43: 179-185 [Abstract] [Full Text]
Tsai, J.-C., Hsueh, P.-R., Lin, H.-M., Chang, H.-J., Ho, S.-W., Teng, L.-J. (2005). Identification of Clinically Relevant Enterococcus Species by Direct Sequencing of groES and Spacer Region. J. Clin. Microbiol. 43: 235-241 [Abstract] [Full Text]
Depardieu, F., Perichon, B., Courvalin, P. (2004). Detection of the van Alphabet and Identification of Enterococci and Staphylococci at the Species Level by Multiplex PCR. J. Clin. Microbiol. 42: 5857-5860 [Abstract] [Full Text]
Kalocheretis, P., Baimakou, E., Zerbala, S., Papaparaskevas, J., Makriniotou, I., Tassios, P. T., Iatrou, C., Kouskouni, E., Zerva, L. (2004). Dissemination of vancomycin-resistant enterococci among haemodialysis patients in Athens, Greece. J Antimicrob Chemother 54: 1031-1034 [Abstract] [Full Text]
Perichon, B., Courvalin, P. (2004). Heterologous Expression of the Enterococcal vanA Operon in Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 48: 4281-4285 [Abstract] [Full Text]
Werner, G., Strommenger, B., Klare, I., Witte, W. (2004). Molecular Detection of Linezolid Resistance in Enterococcus faecium and Enterococcus faecalis by Use of 5' Nuclease Real-Time PCR Compared to a Modified Classical Approach. J. Clin. Microbiol. 42: 5327-5331 [Abstract] [Full Text]
Novais, C., Sousa, J. C., Coque, T. M., Peixe, L. V., on behalf of the Portuguese Resistance Study Group, (2004). In vitro activity of daptomycin against enterococci from nosocomial and community environments in Portugal. J Antimicrob Chemother 54: 964-966 [Full Text]
Titze-de-Almeida, R., Willems, R. J. L., Top, J., Pereira Rodrigues, I., Fonseca Ferreira, R. II, Boelens, H., Brandileone, M. C. C., Zanella, R. C., Soares Felipe, M. S., van Belkum, A. (2004). Multilocus Variable-Number Tandem-Repeat Polymorphism among Brazilian Enterococcus faecalis Strains. J. Clin. Microbiol. 42: 4879-4881 [Abstract] [Full Text]
Novais, C., Coque, T. M., Sousa, J. C., Baquero, F., Peixe, L. (2004). Local Genetic Patterns within a Vancomycin-Resistant Enterococcus faecalis Clone Isolated in Three Hospitals in Portugal. Antimicrob. Agents Chemother. 48: 3613-3617 [Abstract] [Full Text]
Diekema, D. J., Dodgson, K. J., Sigurdardottir, B., Pfaller, M. A. (2004). Rapid Detection of Antimicrobial-Resistant Organism Carriage: an Unmet Clinical Need. J. Clin. Microbiol. 42: 2879-2883 [Full Text]
Sofianou, D., Pournaras, S., Giosi, M., Polyzou, A., Maniatis, A. N., Tsakris, A. (2004). Substantially increased faecal carriage of vancomycin-resistant enterococci in a tertiary Greek hospital after a 4 year time interval. J Antimicrob Chemother 54: 251-254 [Abstract] [Full Text]
David, V., Bozdogan, B., Mainardi, J.-L., Legrand, R., Gutmann, L., Leclercq, R. (2004). Mechanism of Intrinsic Resistance to Vancomycin in Clostridium innocuum NCIB 10674. J. Bacteriol. 186: 3415-3422 [Abstract] [Full Text]
Reynolds, R., Potz, N., Colman, M., Williams, A., Livermore, D., MacGowan, A., on behalf of the BSAC Extended Working Party on Ba, (2004). Antimicrobial susceptibility of the pathogens of bacteraemia in the UK and Ireland 2001-2002: the BSAC Bacteraemia Resistance Surveillance Programme. J Antimicrob Chemother 53: 1018-1032 [Abstract] [Full Text]
Huh, J. Y., Lee, W. G., Lee, K., Shin, W. S., Yoo, J. H. (2004). Distribution of Insertion Sequences Associated with Tn1546-Like Elements among Enterococcus faecium Isolates from Patients in Korea. J. Clin. Microbiol. 42: 1897-1902 [Abstract] [Full Text]
Potz, N. A. C., Mushtaq, S., Johnson, A. P., Henwood, C. J., Walker, R. A., Varey, E., Warner, M., James, D., Livermore, D. M. (2004). Reliability of routine disc susceptibility testing by the British Society for Antimicrobial Chemotherapy (BSAC) method. J Antimicrob Chemother 53: 729-738 [Abstract] [Full Text]
Eom, J.-S., Hwang, I.-S., Hwang, B.-Y., Lee, J.-G., Lee, Y.-J., Cheong, H.-J., Park, Y.-H., Park, S.-C., Kim, W.-J. (2004). Emergence of vanA Genotype Vancomycin-Resistant Enterococci with Low or Moderate Levels of Teicoplanin Resistance in Korea. J. Clin. Microbiol. 42: 1785-1786 [Abstract] [Full Text]
Fluit, A. C., Schmitz, F.-J., Verhoef, J., Milatovic, D. (2004). In Vitro Activity of Daptomycin against Gram-Positive European Clinical Isolates with Defined Resistance Determinants. Antimicrob. Agents Chemother. 48: 1007-1011 [Abstract] [Full Text]
Bujdakova, H., Filipova, M., Liskova, A., Drahovska, H. (2004). Molecular characterization of hospital vancomycin-resistant Enterococcus faecalis isolated in Slovakia. J Antimicrob Chemother 53: 405-406 [Full Text]
Tenover, F. C., Weigel, L. M., Appelbaum, P. C., McDougal, L. K., Chaitram, J., McAllister, S., Clark, N., Killgore, G., O'Hara, C. M., Jevitt, L., Patel, J. B., Bozdogan, B. (2004). Vancomycin-Resistant Staphylococcus aureus Isolate from a Patient in Pennsylvania. Antimicrob. Agents Chemother. 48: 275-280 [Abstract] [Full Text]
Jorgensen, J. H., Crawford, S. A., Kelly, C. C., Patterson, J. E. (2003). In Vitro Activity of Daptomycin against Vancomycin-Resistant Enterococci of Various Van Types and Comparison of Susceptibility Testing Methods. Antimicrob. Agents Chemother. 47: 3760-3763 [Abstract] [Full Text]
Tomita, H., Tanimoto, K., Hayakawa, S., Morinaga, K., Ezaki, K., Oshima, H., Ike, Y. (2003). Highly Conjugative pMG1-Like Plasmids Carrying Tn1546-Like Transposons That Encode Vancomycin Resistance in Enterococcus faecium. J. Bacteriol. 185: 7024-7028 [Abstract] [Full Text]
Routsi, C., Platsouka, E., Willems, R. J. L., Bonten, M. J. M., Paniara, O., Saroglou, G., Roussos, C. (2003). Detection of Enterococcal Surface Protein Gene (esp) and Amplified Fragment Length Polymorphism Typing of Glycopeptide-Resistant Enterococcus faecium during Its Emergence in a Greek Intensive Care Unit. J. Clin. Microbiol. 41: 5742-5746 [Abstract] [Full Text]
Weigel, L. M., Clewell, D. B., Gill, S. R., Clark, N. C., McDougal, L. K., Flannagan, S. E., Kolonay, J. F., Shetty, J., Killgore, G. E., Tenover, F. C. (2003). Genetic Analysis of a High-Level Vancomycin-Resistant Isolate of Staphylococcus aureus. Science 302: 1569-1571 [Abstract] [Full Text]
Nilius, A. M., Shen, L. L., Hensey-Rudloff, D., Almer, L. S., Beyer, J. M., Balli, D. J., Cai, Y., Flamm, R. K. (2003). In Vitro Antibacterial Potency and Spectrum of ABT-492, a New Fluoroquinolone. Antimicrob. Agents Chemother. 47: 3260-3269 [Abstract] [Full Text]
Paule, S. M., Trick, W. E., Tenover, F. C., Lankford, M., Cunningham, S., Stosor, V., Cordell, R. L., Peterson, L. R. (2003). Comparison of PCR Assay to Culture for Surveillance Detection of Vancomycin-Resistant Enterococci. J. Clin. Microbiol. 41: 4805-4807 [Abstract] [Full Text]
Woodford, N., Reynolds, R., Turton, J., Scott, F., Sinclair, A., Williams, A., Livermore, D. (2003). Two widely disseminated strains of Enterococcus faecalis highly resistant to gentamicin and ciprofloxacin from bacteraemias in the UK and Ireland. J Antimicrob Chemother 52: 711-714 [Abstract] [Full Text]
Moubareck, C., Bourgeois, N., Courvalin, P., Doucet-Populaire, F. (2003). Multiple Antibiotic Resistance Gene Transfer from Animal to Human Enterococci in the Digestive Tract of Gnotobiotic Mice. Antimicrob. Agents Chemother. 47: 2993-2996 [Abstract] [Full Text]
Zhanel, G. G., Laing, N. M., Nichol, K. A., Palatnick, L. P., Noreddin, A., Hisanaga, T., Johnson, J. L., the NAVRESS Group, , Hoban, D. J. (2003). Antibiotic activity against urinary tract infection (UTI) isolates of vancomycin-resistant enterococci (VRE): results from the 2002 North American Vancomycin Resistant Enterococci Susceptibility Study (NAVRESS). J Antimicrob Chemother 52: 382-388 [Abstract] [Full Text]
Padiglione, A. A., Wolfe, R., Grabsch, E. A., Olden, D., Pearson, S., Franklin, C., Spelman, D., Mayall, B., Johnson, P. D. R., Grayson, M. L. (2003). Risk Factors for New Detection of Vancomycin-Resistant Enterococci in Acute-Care Hospitals That Employ Strict Infection Control Procedures. Antimicrob. Agents Chemother. 47: 2492-2498 [Abstract] [Full Text]
Dina, J., Malbruny, B., Leclercq, R. (2003). Nonsense Mutations in the lsa-Like Gene in Enterococcus faecalis Isolates Susceptible to Lincosamides and Streptogramins A. Antimicrob. Agents Chemother. 47: 2307-2309 [Abstract] [Full Text]
Jureen, R., Top, J., Mohn, S. C., Harthug, S., Langeland, N., Willems, R. J. L. (2003). Molecular Characterization of Ampicillin-Resistant Enterococcus faecium Isolates from Hospitalized Patients in Norway. J. Clin. Microbiol. 41: 2330-2336 [Abstract] [Full Text]
Palladino, S., Kay, I. D., Flexman, J. P., Boehm, I., Costa, A. M. G., Lambert, E. J., Christiansen, K. J. (2003). Rapid Detection of vanA and vanB Genes Directly from Clinical Specimens and Enrichment Broths by Real-Time Multiplex PCR Assay. J. Clin. Microbiol. 41: 2483-2486 [Abstract] [Full Text]
Critchley, I. A., Blosser-Middleton, R. S., Jones, M. E., Thornsberry, C., Sahm, D. F., Karlowsky, J. A. (2003). Baseline Study To Determine In Vitro Activities of Daptomycin against Gram-Positive Pathogens Isolated in the United States in 2000-2001. Antimicrob. Agents Chemother. 47: 1689-1693 [Abstract] [Full Text]
Critchley, I. A., Draghi, D. C., Sahm, D. F., Thornsberry, C., Jones, M. E., Karlowsky, J. A. (2003). Activity of daptomycin against susceptible and multidrug-resistant Gram-positive pathogens collected in the SECURE study (Europe) during 2000-2001. J Antimicrob Chemother 51: 639-649 [Abstract] [Full Text]
Dutta, I., Reynolds, P. E. (2003). The vanC-3 vancomycin resistance gene cluster of Enterococcus flavescens CCM 439. J Antimicrob Chemother 51: 703-706 [Abstract] [Full Text]
Brown, D. F. J., Walpole, E. (2003). Evaluation of selective and enrichment media for isolation of glycopeptide-resistant enterococci from faecal specimens. J Antimicrob Chemother 51: 289-296 [Abstract] [Full Text]
Simonsen, G. S., Smabrekke, L., Monnet, D. L., Sorensen, T. L., Moller, J. K., Kristinsson, K. G., Lagerqvist-Widh, A., Torell, E., Digranes, A., Harthug, S., Sundsfjord, A. (2003). Prevalence of resistance to ampicillin, gentamicin and vancomycin in Enterococcus faecalis and Enterococcus faecium isolates from clinical specimens and use of antimicrobials in five Nordic hospitals. J Antimicrob Chemother 51: 323-331 [Abstract] [Full Text]
Udo, E. E., Al-Sweih, N., Phillips, O. A., Chugh, T. D. (2003). Species prevalence and antibacterial resistance of enterococci isolated in Kuwait hospitals. J Med Microbiol 52: 163-168 [Abstract] [Full Text]
Arias, C. A., Reyes, J., Zuniga, M., Cortes, L., Cruz, C., Rico, C. L., Panesso, D., on behalf of the Colombian Antimicrobial Resistanc, (2003). Multicentre surveillance of antimicrobial resistance in enterococci and staphylococci from Colombian hospitals, 2001-2002. J Antimicrob Chemother 51: 59-68 [Abstract] [Full Text]
Ozawa, Y., Tanimoto, K., Nomura, T., Yoshinaga, M., Arakawa, Y., Ike, Y. (2002). Vancomycin-Resistant Enterococci in Humans and Imported Chickens in Japan. Appl. Environ. Microbiol. 68: 6457-6461 [Abstract] [Full Text]
Fortun, J., Coque, T. M., Martin-Davila, P., Moreno, L., Canton, R., Loza, E., Baquero, F., Moreno, S. (2002). Risk factors associated with ampicillin resistance in patients with bacteraemia caused by Enterococcus faecium. J Antimicrob Chemother 50: 1003-1009 [Abstract] [Full Text]
Cheng, A. F., Char, T. S., Ling, J. M. (2002). Are multiply resistant enterococci a common phenomenon in Hong Kong?. J Antimicrob Chemother 50: 761-763 [Full Text]
Colak, D., Naas, T., Gunseren, F., Fortineau, N., Ogunc, D., Gultekin, M., Nordmann, P. (2002). First outbreak of vancomycin-resistant enterococci in a tertiary hospital in Turkey. J Antimicrob Chemother 50: 397-401 [Abstract] [Full Text]
Tomita, H., Pierson, C., Lim, S. K., Clewell, D. B., Ike, Y. (2002). Possible Connection between a Widely Disseminated Conjugative Gentamicin Resistance (pMG1-Like) Plasmid and the Emergence of Vancomycin Resistance in Enterococcus faecium. J. Clin. Microbiol. 40: 3326-3333 [Abstract] [Full Text]
Umeda, A., Garnier, F., Courvalin, P., Galimand, M. (2002). Association between the vanB2 glycopeptide resistance operon and Tn1549 in enterococci from France. J Antimicrob Chemother 50: 253-256 [Abstract] [Full Text]
Iversen, A., Kuhn, I., Franklin, A., Mollby, R. (2002). High Prevalence of Vancomycin-Resistant Enterococci in Swedish Sewage. Appl. Environ. Microbiol. 68: 2838-2842 [Abstract] [Full Text]
Dargere, S., Vergnaud, M., Verdon, R., Saloux, E., Le Page, O., Leclercq, R., Bazin, C. (2002). Enterococcus gallinarum Endocarditis Occurring on Native Heart Valves. J. Clin. Microbiol. 40: 2308-2310 [Abstract] [Full Text]
Ligozzi, M., Bernini, C., Bonora, M. G., de Fatima, M., Zuliani, J., Fontana, R. (2002). Evaluation of the VITEK 2 System for Identification and Antimicrobial Susceptibility Testing of Medically Relevant Gram-Positive Cocci. J. Clin. Microbiol. 40: 1681-1686 [Abstract] [Full Text]
Vancanneyt, M., Lombardi, A., Andrighetto, C., Knijff, E., Torriani, S., Bjorkroth, K. J., Franz, C. M. A. P., Foulquie Moreno, M. R., Revets, H., De Vuyst, L., Swings, J., Kersters, K., Dellaglio, F., Holzapfel, W. H. (2002). Intraspecies Genomic Groups in Enterococcus faecium and Their Correlation with Origin and Pathogenicity. Appl. Environ. Microbiol. 68: 1381-1391 [Abstract] [Full Text]
Hancock, L. E., Gilmore, M. S. (2002). The capsular polysaccharide of Enterococcus faecalis and its relationship to other polysaccharides in the cell wall. Proc. Natl. Acad. Sci. USA 99: 1574-1579 [Abstract] [Full Text]
Lauderdale, T.-L., McDonald, L. C., Shiau, Y.-R., Chen, P.-C., Wang, H.-Y., Lai, J.-F., Ho, M. (2002). Vancomycin-Resistant Enterococci from Humans and Retail Chickens in Taiwan with Unique VanB Phenotype-vanA Genotype Incongruence. Antimicrob. Agents Chemother. 46: 525-527 [Abstract] [Full Text]
Novais, C., Vital, C., Ribeiro, G., Coque, T. M., Peixe, L. V. (2002). First characterization of vancomycin-resistant enterococci from a Portuguese hospital.. J Antimicrob Chemother 49: 215-217 [Full Text]
Antignac, A., Alonso, J.-M., Taha, M.-K. (2001). Nonculture Prediction of Neisseria meningitidis Susceptibility to Penicillin. Antimicrob. Agents Chemother. 45: 3625-3628 [Abstract] [Full Text]
Werner, G., Hildebrandt, B., Witte ;, W., Murray, B. E., Singh, K. V. (2001). The Newly Described msrC Gene Is Not Equally Distributed among All Isolates of Enterococcus faecium. Antimicrob. Agents Chemother. 45: 3672-3673 [Full Text]
Kawalec, M., Gniadkowski, M., Kedzierska, J., Skotnicki, A., Fiett, J., Hryniewicz, W. (2001). Selection of a Teicoplanin-Resistant Enterococcus faecium Mutant during an Outbreak Caused by Vancomycin-Resistant Enterococci with the VanB Phenotype. J. Clin. Microbiol. 39: 4274-4282 [Abstract] [Full Text]
Boerlin, P., Wissing, A., Aarestrup, F. M., Frey, J., Nicolet, J. (2001). Antimicrobial Growth Promoter Ban and Resistance to Macrolides and Vancomycin in Enterococci from Pigs. J. Clin. Microbiol. 39: 4193-4195 [Abstract] [Full Text]
Vergis, E. N., Hayden, M. K., Chow, J. W., Snydman, D. R., Zervos, M. J., Linden, P. K., Wagener, M. M., Schmitt, B., Muder, R. R. (2001). Determinants of Vancomycin Resistance and Mortality Rates in Enterococcal Bacteremia: A Prospective Multicenter Study. ANN INTERN MED 135: 484-492 [Abstract] [Full Text]
Fluit, A. C., Visser, M. R., Schmitz, F.-J. (2001). Molecular Detection of Antimicrobial Resistance. Clin. Microbiol. Rev. 14: 836-871 [Abstract] [Full Text]
Teng, L.-J., Hsueh, P.-R., Wang, Y.-H., Lin, H.-M., Luh, K.-T., Ho, S.-W. (2001). Determination of Enterococcus faecalis groESL Full-Length Sequence and Application for Species Identification. J. Clin. Microbiol. 39: 3326-3331 [Abstract] [Full Text]
Elsayed, S., Hamilton, N., Boyd, D., Mulvey, M., Kariyama, R., Mitsuhata, R., Kumon, H. (2001). Improved Primer Design for Multiplex PCR Analysis of Vancomycin-Resistant Enterococcus spp.. J. Clin. Microbiol. 39: 2367-2368 [Full Text]
Butaye, P., Devriese, L. A., Haesebrouck, F. (2001). Differences in Antibiotic Resistance Patterns of Enterococcus faecalis and Enterococcus faecium Strains Isolated from Farm and Pet Animals. Antimicrob. Agents Chemother. 45: 1374-1378 [Abstract] [Full Text]
Kirschner, C., Maquelin, K., Pina, P., Ngo Thi, N. A., Choo-Smith, L.-P., Sockalingum, G. D., Sandt, C., Ami, D., Orsini, F., Doglia, S. M., Allouch, P., Mainfait, M., Puppels, G. J., Naumann, D. (2001). Classification and Identification of Enterococci: a Comparative Phenotypic, Genotypic, and Vibrational Spectroscopic Study. J. Clin. Microbiol. 39: 1763-1770 [Abstract] [Full Text]
Brown, A. R., Townsley, A. C., Amyes, S. G. B. (2001). Diversity of Tn1546 Elements in Clinical Isolates of Glycopeptide-Resistant Enterococci from Scottish Hospitals. Antimicrob. Agents Chemother. 45: 1309-1311 [Abstract] [Full Text]

Antimicrob. Agents Chemother.	Clin. Microbiol. Rev.

Clin. Vaccine Immunol.	ALL ASM JOURNALS