JCM Accepts, published online ahead of print on 14 October 2009

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J. Clin. Microbiol. doi:10.1128/JCM.01696-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Identification of P1 variants of Mycoplasma pneumoniae using High Resolution Melt Analysis

Stephanie B. Schwartz, Stephanie L. Mitchell, Kathleen A. Thurman, Bernard J. Wolff, and Jonas M. Winchell^*

Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA

^* To whom correspondence should be addressed. Email: jwinchell{at}cdc.gov.

Mycoplasma pneumoniae is a leading cause of community-acquired pneumonia. Although two genetically distinct types of M. pneumoniae are known, variants of each also exist. We used a real-time PCR HRM genotyping assay to identify clinical variants which may provide greater insight into the genetic distribution of M. pneumoniae strains.