JCM Accepts, published online ahead of print on 21 October 2009
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J. Clin. Microbiol. doi:10.1128/JCM.01547-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Comparison of PCR and Culture for Screening of Vancomycin-Resistant Enterococci (VRE): Highly Disparate Results for vanA and vanB.

Anton Mak, Mark A. Miller*, George Chong, and Yury Monczak

Department of Microbiology, McGill University Health Centre, Montreal, Quebec; Department of Microbiology, Jewish General Hospital, Montreal, Quebec; Molecular Pathology, Department of Pathology, Jewish General Hospital, Montreal, Quebec

* To whom correspondence should be addressed. Email: mmiller{at}lab.jgh.mcgill.ca.


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Abstract

We compared PCR to conventional culture for the detection of VRE in 30, 835 rectal samples over a three-year period. The positive and negative predictive values of vanB PCR were 1.42% and 99.9%, respectively. A positive vanB result by PCR is poorly predictive and necessitates culture for differentiation of VRE-positive and -negative individuals.






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