JCM Accepts, published online ahead of print on 21 October 2009
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J. Clin. Microbiol. doi:10.1128/JCM.01354-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Incubation Alone is Adequate for Culturing Cardiac Rhythm Management Devices

George M Viola*, Mohammad D. Mansouri, Nadim Nasir Jr, and Rabih O. Darouiche

Department of Medicine, Section of Infectious Diseases, Department of Physical Medicine & Rehabilitation, Baylor College of Medicine, Michael E. DeBakey Veterans Affairs Medical Center and Department of Cardiology, The Methodist Hospital, Houston, Texas, 77030

* To whom correspondence should be addressed. Email: viola{at}bcm.edu.


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Abstract

There exist no standardized methods for culturing cardiac rhythm management devices. To identify the most optimal culturing method, we compared various techniques that comprise vortex, sonication, incubation or combinations thereof. Incubation alone yielded similar bacterial colony counts as other culturing combinations, and is the least labor-intensive.