JCM Accepts, published online ahead of print on 4 November 2009

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J. Clin. Microbiol. doi:10.1128/JCM.01084-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A rapid method for identification of six common Mycobacteria species based on multiplex SNP analysis

Hongxiu Wang, Jun Yue^*, Min Han, Jinghui Yang, and Yanlin Zhao

Key Laboratory of Mycobacteria Tuberculosis, Shanghai Pulmonary Hospital affiliated to Tongji University, Shanghai, 200433, People's Republic of China; Tuberculosis Reference Laboratory, Beijing Thoracic Tumor and Tuberculosis Research Institute, Beijing, 101149, People's Republic of China

^* To whom correspondence should be addressed. Email: yuejunnan{at}yahoo.com.cn.

A multiplex method using the SNaPshot technique was developed to screen for six common mycobacteria species: M.tuberculosis, M.avium, M.intracellulare, M.chelonae, M.kansasii and M.gordonae. A total of 468 mycobacteria clinical isolates were subjected to analysis for the presence of the six mycobacteria species by multiplex SNaPshot method. 464 out of 468 of mycobacteria isolates (99.15%) could be correctly identified by this assay. Multiplex SNaPshot technique is a promising discriminatory tool for rapid and accurate identification of frequently encountered clinical mycobacteria species.