JCM Accepts, published online ahead of print on 28 October 2009

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J. Clin. Microbiol. doi:10.1128/JCM.01015-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Diversity of human parechoviruses isolated in stool samples collected from Thai children with acute gastroenteritis

Ngan Thi Kim Pham, Quang Duy Trinh, Pattara Khamrin, Niwat Maneekarn, Hidaaki Shimizu, Shoko Okitsu, Masashi Mizuguchi, and Hiroshi Ushijima^*

Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; Aino Health Science Center and Aino University, Tokyo, Japan; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Kawasaki City Institute of Health, Kanagawa, Japan; Aino Health Science Center and Aino College, Tokyo, Japan

^* To whom correspondence should be addressed. Email: ushijima-hiroshi{at}jcom.home.ne.jp.

A total of 82 fecal specimens, which had been known to be negative for rotavirus, adenovirus, norovirus, sapovirus, and astrovirus, collected from infants and children with acute gastroenteritis in Chiang Mai, Thailand from January to December 2005, were subjected to screening for human parechovirus (HPeV). HPeV was detected by RT-PCR using a primer pair to amplify the 5'UTR region of its genome and was genotyped by sequencing of the VP1 region. HPeV was detected in 12 of 82 specimens tested, and the detection rate was found to be 14.6%. For the detected HPeV, the capsid VP1 gene of nine strains was successfully sequenced. The studied HPeV strains clustered into 4 different genotypes from HPeV1-4, and the majority of the strains studied (5 strains) belonged to HPeV1. This is the first finding of HPeV from children with acute gastroenteritis in Thailand. In addition, the diversity of Thai HPeV was also noted.