JCM Accepts, published online ahead of print on 21 October 2009
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J. Clin. Microbiol. doi:10.1128/JCM.00176-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Improved selective isolation of Bordetella pertussis by modified cyclodextrin solid medium

Masayuki Ohtsuka, Ken Kikuchi*, Kazuya Shundo, Kenji Okada, Masato Higashide, Keisuke Sunakawa, and Keiichi Hiramatsu

Department of Infection Control Science, Faculty of Medicine, Juntendo University, Kotobiken Medical Laboratories Inc., Nikken Bio Medical Laboratories Inc., Division of Pediatrics, Fukuoka National Hospital, and Department of Infectious Diseases, Kitasato University School of Medicine, Japan

* To whom correspondence should be addressed. Email: kikuti{at}juntendo.ac.jp.


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Abstract

We have developed a modified cyclodextrin solid (MCS) medium using the selective antibiotic, cefdinir. MCS medium exhibited higher sensitivity (95.6%: any culture positive as reference) and greater inhibition of nasopharyngeal flora than Bordet-Gengou agar (65.2%, P = 0.009) or cyclodextrin solid medium (39.1%, P < 0.001).