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JCM Accepts, published online ahead of print on 30 April 2008
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J. Clin. Microbiol. doi:10.1128/JCM.00053-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Detection of caspofungin resistance in Candida spp. by Etest®

Marie Desnos-Ollivier, Françoise Dromer, and Eric Dannaoui*

Centre National de Référence Mycologie et Antifongiques, Unité de Mycologie Moléculaire, CNRS URA3012, Institut Pasteur, 75724 Paris Cedex 15, France; Université Paris Descartes; Faculté de Médecine; AP-HP; Hôpital Européen Georges Pompidou; Unité de Parasitologie – Mycologie, 75015 Paris, France

* To whom correspondence should be addressed. Email: dannaoui{at}pasteur.fr.


  Abstract

Caspofungin susceptibility of 28 Candida spp. clinical isolates, including 8 caspofungin-resistant characterized by mutation in Fks1 protein, was determined by Etest® in RPMI and AM3. A good discrimination between wild-type and mutant isolates was obtained. These results suggest that Etest® is valuable for detection of caspofungin resistance in Candida spp.




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