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Journal of Clinical Microbiology, November 2009, p. 3413-3419, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00136-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Interaction between Host Cells and Septicemic Salmonella enterica Serovar Typhimurium Isolates from Pigs {triangledown}

Nadia Bergeron,1 Jonathan Corriveau,1 Ann Letellier,1 France Daigle,3 Louise Lessard,2 and Sylvain Quessy1*

Department of Pathology and Microbiology, Veterinary Medicine Faculty, Université de Montréal, C.P. 5000, Saint-Hyacinthe, Québec, Canada J2S 7C6,1 Saint-Hyacinthe Laboratory, Canadian Food Inspection Agency, 3400 Boul. Casavant Ouest, Saint-Hyacinthe, Québec, Canada J2S 8E3,2 Department of Microbiology and Immunology, Medicine Faculty, Université de Montréal, C.P. 6128 Branch City Center, Montréal, Québec, Canada H3C 3J73

Received 22 January 2009/ Returned for modification 13 June 2009/ Accepted 13 August 2009

Salmonella enterica serovar Typhimurium is an important pathogen in swine and is also a frequently reported zoonotic agent. The objective of this study was to characterize isolates of S. enterica serovar Typhimurium associated with septicemia in swine and to compare them to isolates recovered from clinically healthy pigs. We were particularly interested in comparing the two groups of isolates for their ability to adhere to and invade host cells, to be phagocytized and survive in monocyte cells, to induce apoptosis, and to adhere to intestinal mucus. Their surface properties were also evaluated by interactions with solvents. The isolates recovered from diseased animals were shown to invade intestinal epithelial cell lines at a higher rate (P = 0.003) than isolates from healthy pigs. Septicemic isolates were phagocytized by human monocytes at a higher rate than isolates from healthy pigs (P = 0.009). The mean percentages of phagocytosis were significantly lower for human monocytes than for porcine monocytes (P = 0.02 and P = 0.008, respectively) for isolates from both diseased and healthy animals. Healthy animal isolates were phagocytized more by porcine monocytes at 15 min (P = 0.02) than septicemic isolates. No difference between isolates from septicemic pigs and isolates from healthy pigs was detected for other tested parameters. These results suggest that septicemic isolates have a particular pattern of invasion.

* Corresponding author. Mailing address: Department of Pathology and Microbiology, Veterinary Medicine Faculty, Université de Montréal, C.P. 5000, Saint-Hyacinthe, Québec, Canada J2S 7C6. Phone: (450) 773-8521, ext. 8398. Fax: (450) 778-8157. E-mail: sylvain.quessy{at}umontreal.ca

{triangledown} Published ahead of print on 26 August 2009.

Journal of Clinical Microbiology, November 2009, p. 3413-3419, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00136-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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