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Journal of Clinical Microbiology, April 2005, p. 1934-1936, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1934-1936.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison between rpoB and 16S rRNA Gene Sequencing for Molecular Identification of 168 Clinical Isolates of Corynebacterium

Atieh Khamis, Didier Raoult, and Bernard La Scola*

Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerrannée, Marseille, France

Received 4 August 2004/ Returned for modification 6 October 2004/ Accepted 16 December 2004

Higher proportions (91%) of 168 corynebacterial isolates were positively identified by partial rpoB gene determination than by that based on 16S rRNA gene sequences. This method is thus a simple, molecular-analysis-based method for identification of corynebacteria, but it should be used in conjunction with other tests for definitive identification.


* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerrannée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France. Phone: 33.04.91.38.55.17. Fax: 33.04.91.83.03.90. E-mail: bernard.lascola{at}medecine.univ-mrs.fr.


Journal of Clinical Microbiology, April 2005, p. 1934-1936, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1934-1936.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




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